Reproduction Abstracts

Introduction: This work adapts a novel ovary culture technique, devised by De Felici’s group, for assessing the ovotoxicity of two chemotherapeutic drugs, cisplatin and doxorubicin. Previous work from our laboratory used cultures of intact neonatal ovaries to determine the effects of the two drugs, showing that both cisplatin and doxorubicin are moderately ovotoxic.Methods: Here, ovaries collected from newborn WT mice were cultured as by De Felici. ...

The past few decades have seen marked improvements in life expectancy following childhood cancer due, in part, to advances in chemotherapy. While these drugs are effective in treating malignant disease, one of the main adverse outcomes can be infertility. This is of particular concern for prepubertal boys, for whom there are not yet any established methods of fertility preservation. The objective of this study was to gain a better understanding of the effects of the widely-use...

Introduction: The effect of chemotherapy treatment on premenopausal women’s fertility has long been of concern. Here, we investigate the direct actions of chemotherapy drugs on the human ovary, examining effects of two commonly used drugs, cisplatin and doxorubicin.Method: Ovarian cortical tissue samples were collected from patients aged 27–34 who were undergoing elective caesarean section, with informed consent and Ethical Committee approval. ...